Publications:

• Crawford MJ, Thomsen-Zieger N, Ray M, Schachtner J, Roos DS, Seeber F. Toxoplasma gondii scavenges host-derived lipoic acid despite its de novo synthesis in the apicoplast. EMBO J. 2006 Jun 15; [Epub ahead of print]
• Hu K, Johnson J, Florens L, Fraunholz M, Suravajjala S, Dilullo C, Yates J, Roos DS, Murray JM. Cytoskeletal Components of an Invasion Machine-The Apical Complex of Toxoplasma gondii. PLoS Pathog. 2006 Feb;2(2):e13. Epub 2006 Feb 24. PMID: 16518471 [PubMed - in process]
• Chen F, Mackey AJ, Stoeckert CJ Jr, Roos DS. OrthoMCL-DB: querying a comprehensive multi-species collection of ortholog groups.Nucleic Acids Res. 2006 Jan 1;34(Database issue):D363-8. PMID: 16381887 [PubMed - indexed for MEDLINE]
• Radke JR, Behnke MS, Mackey AJ, Radke JB, Roos DS, White MW. The transcriptome of Toxoplasma gondii.
  BMC Biol. 2005 Dec 2;3:26. PMID: 16324218 [PubMed - in process]
• Hartmann J, Hu K, He CY, Pelletier L, Roos DS, Warren G. Golgi and centrosome cycles in Toxoplasma gondii.
  Mol Biochem Parasitol. 2006 Jan;145(1):125-7. Epub 2005 Oct 17. No abstract available. PMID: 16266757 [PubMed - indexed for MEDLINE]
  
• Chaudhary K, Roos DS. Protozoan genomics for drug discovery. Nat Biotechnol. 2005 Sep;23(9):1089-91 PMID: 16151400
• Shapira S, Harb OS, Margarit J, Matrajt M, Han J, Hoffmann A, Freedman B, May MJ, Roos DS, Hunter CA. Initiation and termination of NF-kappaB signaling by the intracellular protozoan parasite Toxoplasma gondii. J Cell Sci. 2005 Aug 1;118(Pt 15):3501-8. PMID: 16079291
• Echeverria PC, Matrajt M, Harb OS, Zappia MP, Costas MA, Roos DS, Dubremetz JF, Angel SO. Toxoplasma gondii Hsp90 is a potential drug target whose expression and subcellular localization are developmentally regulated. J Mol Biol. 2005 Jul 22;350(4):723-34. PMID: 15967463
• Roos DS. Genetics. Themes and variations in apicomplexan parasite biology. Science. 2005 Jul 1;309(5731):72-3. No abstract available. PMID: 15994520
• Chaudhary K, Donald RG, Nishi M, Carter D, Ullman B, Roos DS. Differential localization of alternatively spliced hypoxanthine-xanthine-guanine phosphoribosyltransferase isoforms in Toxoplasma gondii. J Biol Chem. 2005 Jun 10;280(23):22053-9. Epub 2005 Apr 6. PMID: 15814612
• Khan A, Taylor S, Su C, Mackey AJ, Boyle J, Cole R, Glover D, Tang K, Paulsen IT, Berriman M, Boothroyd JC, Pfefferkorn ER, Dubey JP, Ajioka JW, Roos DS, Wootton JC, Sibley LD. Composite genome map and recombination parameters derived from three archetypal lineages of Toxoplasma gondii. Nucleic Acids Res. 2005 May 23;33(9):2980-92. Print 2005. PMID: 15911631
• Pepper M, Dzierszinski F, Crawford A, Hunter CA, Roos D. Free in PMC Development of a system to study CD4+-T-cell responses to transgenic ovalbumin-expressing Toxoplasma gondii during toxoplasmosis. Infect Immun. 2004 Dec;72(12):7240-6. PMID: 15557649 [PubMed - indexed for MEDLINE]
• Tongren JE, Zavala F, Roos DS, Riley EM. Malaria vaccines: if at first you don't succeed... Trends Parasitol. 2004 Dec;20(12):604-10. Review. PMID: 15522671
• Coppel RL, Roos DS, Bozdech Z. The genomics of malaria infection. Trends Parasitol. 2004 Dec;20(12):553-7. Review. PMID: 15522663
• Berry AE, Gardner MJ, Caspers GJ, Roos DS, Berriman M. Curation of the Plasmodium falciparum genome. Trends Parasitol. 2004 Dec;20(12):548-52. Review. PMID: 15522662
• Hu K, Roos DS, Angel SO, Murray JM. Variability and heritability of cell division pathways in Toxoplasma gondii. J Cell Sci. 2004 Nov 1;117(Pt 23):5697-705. Epub 2004 Oct 19. PMID: 15494366
• Matrajt M, Platt CD, Sagar AD, Lindsay A, Moulton C, Roos DS. Transcript initiation, polyadenylation, and functional promoter mapping for the dihydrofolate reductase-thymidylate synthase gene of Toxoplasma gondii. Mol Biochem Parasitol. 2004 Oct;137(2):229-38. PMID: 15383293
• McKee AS, Dzierszinski F, Boes M, Roos DS, Pearce EJ. Functional inactivation of immature dendritic cells by the intracellular parasite Toxoplasma gondii. J Immunol. 2004 Aug 15;173(4):2632-40. PMID: 15294980
• Dzierszinski F, Nishi M, Ouko L, Roos DS. Dynamics of Toxoplasma gondii differentiation. Eukaryot Cell. 2004 Aug;3(4):992-1003. PMID: 15302832
• Chaudhary K, Darling JA, Fohl LM, Sullivan WJ Jr, Donald RG, Pfefferkorn ER, Ullman B, Roos DS. Purine salvage pathways in the apicomplexan parasite Toxoplasma gondii. J Biol Chem. 2004 Jul 23;279(30):31221-7. Epub 2004 May 11. PMID: 15140885
• Stwora-Wojczyk MM, Dzierszinski F, Roos DS, Spitalnik SL, Wojczyk BS. Functional characterization of a novel Toxoplasma gondii glycosyltransferase: UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-T3. Arch Biochem Biophys. 2004 Jun 15;426(2):231-40. PMID: 15158673
• Harb OS, Chatterjee B, Fraunholz MJ, Crawford MJ, Nishi M, Roos DS. Multiple functionally redundant signals mediate targeting to the apicoplast in the apicomplexan parasite Toxoplasma gondii. Eukaryot Cell. 2004 Jun;3(3):663-74. PMID: 15189987
• Wille U, Nishi M, Lieberman L, Wilson EH, Roos DS, Hunter CA. IL-10 is not required to prevent immune hyperactivity during memory responses to Toxoplasma gondii. Parasite Immunol. 2004 May;26(5):229-36. PMID: 15491472
• Ralph SA, van Dooren GG, Waller RF, Crawford MJ, Fraunholz MJ, Foth BJ, Tonkin CJ, Roos DS, McFadden GI. Tropical infectious diseases: metabolic maps and functions of the Plasmodium falciparum apicoplast. Nat Rev Microbiol. 2004 Mar;2(3):203-16. Review. No abstract available. PMID: 15083156
• Li L, Crabtree J, Fischer S, Pinney D, Stoeckert CJ Jr, Sibley LD, Roos DS. ApiEST-DB: analyzing clustered EST data of the apicomplexan parasites. Nucleic Acids Res. 2004 Jan 1;32(Database issue):D326-8. PMID: 14681425
• Fohl LM, Roos DS. Fitness effects of DHFR-TS mutations associated with pyrimethamine resistance in apicomplexan parasites. Mol Microbiol. 2003 Nov;50(4):1319-27. PMID: 14622418
• Wojczyk BS, Stwora-Wojczyk MM, Hagen FK, Striepen B, Hang HC, Bertozzi CR, Roos DS, Spitalnik SL. cDNA cloning and expression of UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase T1 from Toxoplasma gondii. Mol Biochem Parasitol. 2003 Oct;131(2):93-107. PMID: 14511808
• Li L, Stoeckert CJ Jr, Roos DS. OrthoMCL: identification of ortholog groups for eukaryotic genomes. Genome Res. 2003 Sep;13(9):2178-89. PMID: 12952885
• Li L, Brunk BP, Kissinger JC, Pape D, Tang K, Cole RH, Martin J, Wylie T, Dante M, Fogarty SJ, Howe DK, Liberator P, Diaz C, Anderson J, White M, Jerome ME, Johnson EA, Radke JA, Stoeckert CJ Jr, Waterston RH, Clifton SW, Roos DS, Sibley LD. (2003) Gene discovery in the apicomplexa as revealed by EST sequencing and assembly of a comparative gene database. Genome Res. 13(3):443-454. PMID: 12618375


• Foth BJ, Ralph SA, Tonkin CJ, Struck NS, Fraunholz M, Roos DS, Cowman AF, McFadden GI. (2003) Dissecting apicoplast targeting in the malaria parasite Plasmodium falciparum. Science. 299(5607):705-708. PMID: 12560551


• Kissinger JC, Gajria B, Li L, Paulsen IT, Roos DS. (2003) ToxoDB: accessing the Toxoplasma gondii genome. Nucleic Acids Res. 31(1):234-236. PMID: 12519989


• Bahl A, Brunk B, Crabtree J, Fraunholz MJ, Gajria B, Grant GR, Ginsburg H, Gupta D, Kissinger JC, Labo P, Li L, Mailman MD, Milgram AJ, Pearson DS, Roos DS, Schug J, Stoeckert CJ Jr, Whetzel P. (2003) PlasmoDB: the Plasmodium genome resource. A database integrating experimental and computational data. Nucleic Acids Res. 31(1):212-215. PMID: 12519984


• Drozdowicz YM, Shaw M, Nishi M, Striepen B, Liwinski HA, Roos DS, Rea PA. (2003) Isolation and characterization of TgVP1, a type I vacuolar H+-translocating pyrophosphatase from Toxoplasma gondii. The dynamics of its subcellular localization and the cellular effects of a diphosphonate inhibitor. J Biol Chem. 278(2):1075-1085. PMID: 12411435


• Gardner MJ, Hall N, Fung E, White O, Berriman M, Hyman RW, Carlton JM, Pain A, Nelson KE, Bowman S, Paulsen IT, James K, Eisen JA, Rutherford K, Salzberg SL, Craig A, Kyes S, Chan MS, Nene V, Shallom SJ, Suh B, Peterson J, Angiuoli S, Pertea M, Allen J, Selengut J, Haft D, Mather MW, Vaidya AB, Martin DM, Fairlamb AH, Fraunholz MJ, Roos DS, Ralph SA, McFadden GI, Cummings LM, Subramanian GM, Mungall C, Venter JC, Carucci DJ, Hoffman SL, Newbold C, Davis RW, Fraser CM, Barrell B. (2002) Genome sequence of the human malaria parasite Plasmodium falciparum. Nature. 419(6906):498-511. PMID: 12368864


• Kissinger JC, Brunk BP, Crabtree J, Fraunholz MJ, Gajria B, Milgram AJ, Pearson DS, Schug J, Bahl A, Diskin SJ, Ginsburg H, Grant GR, Gupta D, Labo P, Li L, Mailman MD, McWeeney SK, Whetzel P, Stoeckert CJ, Roos DS.(2002) The Plasmodium genome database. Nature. 419(6906):490-492. PMID: 12368860


• Pelletier, L., Stern, C.A., Pypaert, M., Sheff, D., Ngo., H.M., Roper, N., He, C.Y., Hu, K., Toomre, D., Coppens, I., Roos, D.S., Joiner, K.A., Warren, G. (2002) Golgi biogenesis in Toxoplasma gondii. Nature. 418:548-552. PMID: 12152082


• Joiner, K.A., Roos, D.S. (2002) Secretory traffic in the eukaryotic parasite Toxoplasma gondii: less is more. J Cell Biol. 157:557-563. Abstract PMID: 12011107


• Matrajt, M., Donald, R.G., Singh, U., Roos, D.S. (2002) Identification and characterization of differentiation mutants in the protozoan parasite Toxoplasma gondii. Mol Microbiol. 44:735-747. Abstract (PMID: 11994154)


• Matrajt, M., Nishi, M., Fraunholz, M.J., Peter, O., Roos, D.S. (2002). Amino-terminal control of transgenic protein expression levels in Toxoplasma gondii. Mol. Biochem. Parasitol. 120:285-289. Abstract (PMID:11897133)


• Hu, K., Roos, D.S., Murray, J.M. (2002) A novel polymer of tubulin forms the conoid of Toxoplasma gondii. J. Cell Biol. 156:1039-1050. Abstract (PMID:11901169)


• Hu K., Mann T., Striepen B., Beckers C.J., Roos D.S., Murray J.M. (2002) Daughter Cell Assembly in the Protozoan Parasite Toxoplasma gondii. Mol Biol Cell. 13:593-606. Abstract (PMID:11854415)


• Roos, D.S., Crawford, M.J., Donald, R.G., Fraunholz, M., Harb, O.S., He, C.Y., Kissinger, J.C., Shaw, M.K., Striepen, B. (2002) Mining the Plasmodium genome database to define organellar function: what does the apicoplast do? Philos. Trans. R. Soc. Lond. B. Biol. Sci. 357:35-46. Abstract (PMID:11839180)


• Swedlow, J.R., Hu, K., Andrews, P.D., Roos, D.S., Murray, J.M. (2002) Measuring tubulin content in Toxoplasma gondii: A comparison of laser-scanning confocal and wide-field fluorescence microscopy. Proc. Natl. Acad. Sci. USA. 99:2014-2019. Abstract (PMID:11830634)


• Bahl, A., Brunk, B., Coppel, R.L., Crabtree, J., Diskin, S.J., Fraunholz, M.J., Grant, G.R., Gupta, D., Huestis, R.L., Kissinger, J.C., Labo, P., Li, L., McWeeney, S.K., Milgram, A.J., Roos, D.S., Schug, J., Stoeckert, C.J. Jr. (2002) PlasmoDB: the Plasmodium genome resource. An integrated database providing tools for accessing, analyzing and mapping expression and sequence data (both finished and unfinished). Nucleic Acids Res. 30:87-90. Abstract (PMID:11752262)


• Radke, J.R., Striepen, B., Guerini, M.N., Jerome, M.E., Roos, D.S., White, M.W. (2001) Defining the cell cycle for the tachyzoite stage of Toxoplasma gondii. Mol. Biochem. Parasitol. 115:165-175. Abstract (PMID:11420103)


• Wille, U., Villegas, E.N., Striepen, B., Roos, D.S., Hunter, C.A. (2001) Interleukin-10 does not contribute to the pathogenesis of a virulent strain of Toxoplasma gondii. Parasite Immunol. 23:291-296. Abstract (PMID:11412381)


• Shaw, M.K., Roos, D.S., Tilney, L.G. (2001) DNA replication and daughter cell budding are not tightly linked in the protozoan parasite Toxoplasma gondii. Microbes Infect. 3:351-62. Abstract (PMID:11369271)


• He, C.Y., Striepen, B., Pletcher, C.H., Murray, J.M., Roos, D.S. (2001) Targeting and processing of nuclear-encoded apicoplast proteins in plastid segregation mutants of Toxoplasma gondii. J. Biol. Chem. 276:28436-42. Abstract (PMID:11319231)


• Akopyants, N.S., Clifton, S.W., Martin, J., Pape, D., Wylie, T., Li, L., Kissinger, J.C., Roos D.S., Beverley, S.M. (2001) A survey of the Leishmania major Friedlin strain V1 genome by shotgun sequencing: a resource for DNA microarrays and expression profiling. Mol. Biochem. Parasitol. 113:337-340. Abstract (PMID:11295190)


• Reynolds, M.G., Oh, J., Roos, D.S. (2001) In vitro generation of novel pyrimethamine resistance mutations in the Toxoplasma gondii dihydrofolate reductase. Antimicrob. Agents Chemother. 45:1271-7. Abstract (PMID:11257045)


• Striepen, B., Soldati, D., Garcia-Reguet, N., Dubremetz, J.F., Roos, D.S. (2001) Targeting of soluble proteins to the rhoptries and micronemes in Toxoplasma gondii. Mol. Biochem. Parasitol. 113:45-53. Abstract (PMID:11254953)


• Roos, D.S. (2001). Computational biology. Bioinformatics--trying to swim in a sea of data. Science. 291:1260-1261. Abstract (PMID:11233452)


• Fast, N.M., Kissinger, J.C., Roos, D.S., Keeling, P.J. (2001) Nuclear-encoded, plastid-targeted genes suggest a single common origin for apicomplexan and dinoflagellate plastids. Mol. Biol. Evol. 18:418-426. Abstract (PMID:11230543)


• Jelenska, J., Crawford, M.J., Harb, O.S., Zuther, E., Haselkorn, R., Roos, D.S., Gornicki, P. (2001) Subcellular localization of acetyl-CoA carboxylase in the apicomplexan parasite Toxoplasma gondii. Proc. Natl. Acad. Sci. USA 98:2723-8. Abstract (PMID:11226307)


• He, C.Y., Shaw, M.K., Pletcher, C.H., Striepen, B., Tilney, L.G., Roos, D.S. (2001) A plastid segregation defect in the protozoan parasite Toxoplasma gondii. EMBO J. 20:330-339. Abstract (PMID:11157740)


• The Plasmodium Genome Database Collaborative. (2001). PlasmoDB: An integrative database of the Plasmodium falciparum genome. Tools for accessing and analyzing finished and unfinished sequence data. Nucleic Acids Res. 29:66-69. Abstract (PMID:11125051)


• Striepen, B., Crawford M.J., Shaw, M.K., Tilney, L.G., Seeber, F., Roos, D.S. (2000). The plastid of Toxoplasma gondii is divided by association with the centrosomes. J. Cell Biol. 151:1423-34. Abstract (PMID:11134072)


• Shaw, M.K., He, C.Y., Roos, D.S., Tilney, L.G. (2000) Proteasome inhibitors block intracellular growth and replication of Toxoplasma gondii. Parasitology. 121:35-47. Abstract (PMID:11085223)


• van Dooren, G.G., Waller, R.F., Joiner, K.A., Roos, D.S., McFadden, G.I. (2000) Traffic jams: protein transport in Plasmodium falciparum. Parasitol. Today. 16:421-7. Abstract (PMID:11006473)


• Baldi, D.L., Andrews, K.T., Waller, R.F., Roos, D.S., Howard, R.F., Crabb, B.S., Cowman, A.F. (2000) RAP1 controls rhoptry targeting of RAP2 in the malaria parasite Plasmodium falciparum. EMBO J. 19:2435-2443. Abstract (PMID:10835342)


• Schumacher MA, Scott DM, Mathews II, Ealick SE, Roos DS, Ullman B, Brennan RG. 2000. Crystal structures of Toxoplasma gondii adenosine kinase reveal a novel catalytic mechanism and prodrug binding. J Mol Biol. 298:875-93. Abstract (PMID:10801355)


• Shaw, M.K., Compton, H.L., Roos, D.S., Tilney, L.G. (2000) Microtubules, but not actin filaments, drive daughter cell budding and cell division in Toxoplasma gondii. J Cell Sci. 113:1241-1254. Abstract (PMID:10704375)


• Schumacher, M.A., Scott, D.M., Mathews, I.I., Ealick, S.E., Roos, D.S., Ullman, B., Brennan, R.G. (2000) Crystal structures of Toxoplasma gondii adenosine kinase reveal a novel catalytic mechanism and prodrug binding. J Mol Biol. 296:549-567. Abstract (PMID:10669608)


• Roos, D.S., Crawford, M.J., Donald, R.G., Fohl, L.M., Hager, K.M., Kissinger, J.C., Reynolds, M.G., Striepen, B., Sullivan, W.J. Jr. (1999) Transport and trafficking: Toxoplasma as a model for Plasmodium. Novartis Found Symp. 226:176-195; discussion 195-198Abstract (PMID:10645546)


• Chiang, C.W., Carter, N., Sullivan, W.J. Jr., Donald, R.G., Roos, D.S., Naguib, F.N., el Kouni, M.H., Ullman, B., Wilson, C.M. (1999) The adenosine transporter of Toxoplasma gondii. Identification by insertional mutagenesis, cloning, and recombinant expression. J Biol Chem. 274:35255-35261. Abstract (PMID:10575012)


• Darling, J.A., Sullivan, W.J. Jr., Carter, D., Ullman, B., Roos, D.S. (1999) Recombinant expression, purification, and characterization of Toxoplasma gondii adenosine kinase. Mol Biochem Parasitol. 103:15-23. Abstract (PMID:10514077)


• Sullivan, W.J. Jr., Chiang, C.W., Wilson, C.M., Naguib, F.N., el Kouni, M.H., Donald, R.G., Roos, D.S. (1999) Insertional tagging of at least two loci associated with resistance to adenine arabinoside in Toxoplasma gondii, and cloning of the adenosine kinase locus. Mol Biochem Parasitol. 103:1-14. Abstract (PMID:10514076)


• Roos, D.S., Crawford, M.J., Donald, R.G., Kissinger, J.C., Klimczak, L.J., Striepen, B. (1999) Origin, targeting, and function of the apicomplexan plastid. Curr Opin Microbiol. 2:426-432. Abstract (PMID:10458993)


• McFadden, G.I., Roos, D.S. (1999) Apicomplexan plastids as drug targets. Trends Microbiol. 7:328-333. Abstract (PMID:10431206)


• Hager, K.M., Striepen, B., Tilney, L.G., Roos, D.S. (1999) The nuclear envelope serves as an intermediary between the ER and Golgi complex in the intracellular parasite Toxoplasma gondii. J Cell Sci. 112:2631-2638. Abstract (PMID:10413671)


• Roos, D.S. (1999) The apicoplast as a potential therapeutic target in Toxoplasma and other apicomplexan parasites: some additional thoughts. Parasitol Today 15:41. Abstract (PMID:10234180)


• Matrajt, M., Angel, S.O., Pszenny, V., Guarnera, E., Roos, D.S., Garberi, J.C. (1999) Arrays of repetitive DNA elements in the largest chromosomes of Toxoplasma gondii. Genome 42:265-269.


• Keeling, P.J., J.D. Palmer, R.G.K. Donald, D.S. Roos, R.F. Waller, and G.I. McFadden. 1999. Shikimate in apicomplexa ... but not in the plastid. Nature 397:219-220.


• Chaturvedi, S., H. Qi, D. Coleman, A. Rodriguez, P.S. Hanson, B. Striepen, D.S. Roos, and K.A. Joiner. 1999. Constitutive calcium independent release of Toxoplasma gondii dense granules occurs through the NSF/SNAP/SNARE/Rab machinery. J. Biol. Chem. 274:2424-2431.


• Roos, D.S. 1999. The apicoplast as a potential therapeutic target in Toxoplasma and other apicomplexan parasites. Parasitol. Today 15:41.


• Shaw, M.W., D.S. Roos, and L.G. Tilney. 1998. Acidic compartments and rhoptry formation in Toxoplasma gondii. Parasitology 117:435-443.


• Roos, D.S., and C.F. Delwiche. 1998. Endosymbiosis: cyclical and permanent in evolution (Response). Trends Microbiol., 6:345-346.


• Waller, R.F., P.J. Keeling, R.G.K. Donald, B. Striepen, E. Handman, N. Lang-Unnasch, A.F. Cowman, G.S. Besra, D.S. Roos, and G.I. McFadden. 1998. Nuclear-encoded proteins target to the plastid in Toxoplasma gondii and Plasmodium falciparum. Proc. Nat'l Acad. Sci. USA 95:12352-12357.


• Jerome, M.E., J.R. Radke, W. Bohne, D.S. Roos, and M.W. White. 1998. Toxoplasma gondii bradyzoites form spontaneously during sporozoite-initiated development: Regulation of tachyzoite growth is linked to stage conversion. Infect. Immun. 66:4838-4844.


• Roos, D.S. 1998. Toxoplasma gondii: An appealing system for exploring parasite biology. Austral. Biochem. 29:2-5.


• Morrissette, N.S., and D.S. Roos. 1998. Toxoplasma gondii: A family of apical antigens associated with the cytoskeleton. Exper. Parasitol., 89:296-303.


• Schumacher, M.A., D. Carter, D.M. Scott, D.S. Roos, B. Ullman, and R.G. Brennan. 1998. Crystal structures of Toxoplasma gondii uracil phosphoribosyltransferase reveal the atomic structure of pyrimidine discrimination and prodrug binding. EMBO J. 17:3219-3232.


• Striepen, B., C.Y. He, M. Matrajt, D. Soldati, and D.S. Roos. 1998. Expression, selection, and organellar targeting of the Green Fluorescent Protein in Toxoplasma gondii. Molec. Biochem. Parasitol. 92:328-338.


• Bohne, W., C.A. Hunter, M.W. White, D.J.P. Ferguson, U. Gross and D.S. Roos. 1998. Targeted disruption of the bradyzoite-specific gene BAG1 does not prevent tissue cyst formation in Toxoplasma gondii. Molec. Biochem. Parasitol. 92:291-301.


• Donald, R.G.K., and D.S. Roos. 1998. Gene knock-outs and allelic replacements in Toxoplasma gondii: HXGPRT as a selectable marker for 'hit-and-run' mutagenesis. Molec. Biochem. Parasitol. 91:295-305.


• Reynolds, M.G., and D.S. Roos. 1998. A biochemical and genetic model for parasite resistance to antifolates. Toxoplasma gondii provides insights into pyrimethamine and cycloguanil resistance in Plasmodium falciparum. J. Biol. Chem. 273:3461-3469.


• Ajioka, J., J.C. Boothroyd, B.P. Brunk, A. Hehl, L. Hillier, I.D. Manger, G.C. Overton, M. Marra, D.S. Roos, K.L. Wan, R. Waterston and L.D. Sibley. 1998. Gene discovery by EST sequencing in Toxoplasma gondii reveals sequences restricted to the apicomplexa. Genome Research 8:18-28.


• Roos, D.S., W.J. Sullivan, B. Striepen, W. Bohne and R.G.K. Donald. 1997. Tagging genes and trapping promoters in Toxoplasma gondii by insertional mutagenesis. Methods 13:112-122.


• Fichera, M.E., and D.S. Roos. 1997. A plastid organelle as a drug target in apicomplexan parasites. Nature 389:407-409.


• Tilley, M., M.E. Fichera, M.E. Jerome, D.S. Roos and M.W. White. 1997. Morphological, immunological, and functional criteria distinguish the two parasitophorous vacuoles formed sequentially during invasion by Toxoplasma gondii sporozoites. Infect. Immun. 65:4598-4605.


• Roos, D.S. 1997. Toxoplasma gondii -- a genetic model for the investigation of drug-resistance mechanisms, parasite differentiation, and the evolution of eukaryotic organelles. Mem. Inst. Oswaldo Cruz. 92:9-10.


• Bohne, W., and D.S. Roos. 1997. Stage-specific expression of a selectable marker in Toxoplasma gondii permits selective inhibition of either tachyzoites or bradyzoites. Molec. Biochem. Parasitol. 88:115-126.


• Carter, D., R.G.K. Donald, D.S. Roos and B. Ullman. 1997. Expression, purification, and characterization of uracil phosphoribosyltransferase from Toxoplasma gondii Molec. Biochem. Parasitol. 87:137-144.


• Brunk, B.P., W. Bohne, G.C. Overton and D.S. Roos. 1997. Mining data from dbEST -- querying the Toxoplasma gondii expressed sequence tag (EST) database (web-site). http://daphne.humgen.upenn.edu:1024/toxodb/ver_2/toxodb.html


• Köhler, S., C.F. Delwiche, P.W. Denny, L.G. Tilney, P. Webster, R.J.M. Wilson, J.D. Palmer and D.S. Roos. 1997. A plastid of probable green algal origin in apicomplexan parasites. Science 275:1485-1488.


• Morrissette, N.S., J.M. Murray and D.S. Roos. 1997. A subpellicular lattice in the protozoan parasite Toxoplasma gondii: Possible mechanisms for motility in the Apicomplexa. J. Cell Sci. 110:35-42.


• Lindsay, D.S., and D.S. Roos. 1996. Current status of research on Toxoplasma gondii. J. Eukaryotic Microbiol. 43:125-127.


• Schumacher, M.A., D. Carter, D.S. Roos, B. Ullman, and R.G. Brennan. 1996. Crystal structures of Toxoplasma gondii HXGPRTase reveal the catalytic role of a long flexible loop. Nature (Struct. Biol.). 3:881-887.


• Stokkermans, T.J.W., J.D. Schwartzman, K. Keenan, N.S. Morrissette., L.G. Tilney, and D.S. Roos. 1996. Inhibition of Toxoplasma gondii replication by dinitroaniline herbicides. Exper. Parasitol. 84:355-370.


• Donald, R.G.K., D. Carter, B. Ullman and D.S. Roos. 1996. Insertional tagging, cloning and expression of the Toxoplasma gondii hypoxanthine-xanthine-guanine phosphoribosyl transferase gene: Characterization of a new genetic marker for stable transformation. J. Biol. Chem. 271:14010-14019.


• Trujillo, M., R.G.K. Donald, D.S. Roos, P.J. Greene and D.V. Santi. 1996. Heterologous expression and characterization of the bifunctional dihydrofolate reductase-thymidylate synthase enzyme of Toxoplasma gondii. Biochemistry 35:6366-6374.


• Roos, D.S. 1996. Molecular genetic tools for the identification and analysis of drug targets in Toxoplasma gondii. Current Topics Microbiol. Immunol. 219:239-251.


• Schlichterle, I.M., D.S. Roos and J.L. van Houten. 1996. Cloning and molecular analysis of the bifunctional dihydrofolate reductase-thymidylate synthase gene in the ciliated protozoan Paramecium tetraurelia. Molec. Gen. Genet. 250:665-673.


• Roos, D.S. 1995. A biologist's perspective on interesting questions and practical difficulties in phylogeny reconstruction. DIMACS Tech. Rep. 95-48:42-47.


• Fichera, M.E., M.K. Bhopale, and D.S. Roos. 1995. In vitro assays elucidate the peculiar mechanism of macrolide/lincosamide action against Toxoplasma gondii. Antimicr. Agents Chemother. 39:1530-1537.


• Donald, R.G.K., and D.S. Roos. 1995. Insertional mutagenesis in a protozoan parasite: Direct cloning of the uracil phosphoribosyl transferase gene from Toxoplasma gondii. Proc. Nat'l Acad. Sci. 92:5749-5753.


• Beckers, C.J.M, D.S. Roos, R.G.K. Donald, B.J. Luft, J.C. Schwab, Y. Cao and K.A. Joiner. 1995. Inhibition of cytoplasmic and organellar protein synthesis in Toxoplasma gondii: Implications for the target of macrolide antibiotics. J. Clin. Invest. 95:367-376.


• Roos, D.S., R.G.K. Donald, N.S. Morrissette and A.L.C. Moulton. 1994. Molecular tools for genetic dissection of the protozoan parasite Toxoplasma gondii. Methods Cell Biol. 45:27-63.


• Morrissette, N.S., P. Webster, V. Bedian and D.S. Roos. 1994. Characterization of extreme apical antigens from Toxoplasma gondii. Exper. Parasitol. 79:445-459.


• Donald, R.G.K., and D.S. Roos. 1994. Homologous recombination and gene replacement at the dihydrofolate reductase/thymidylate synthase locus in Toxoplasma gondii. Molec. Biochem. Parasitol. 63:243-253.


• Donald, R.G.K., and D.S. Roos. 1993. Stable molecular transformation of Toxoplasma gondii: A selectable dihydrofolate reductase/thymidylate synthase marker based on drug resistance mutations in malaria. Proc. Nat'l Acad. Sci. USA 90:11703-11707.


• Roos, D.S. 1993. Primary structure of the dihydrofolate reductase/thymidylate synthase gene from Toxoplasma gondii. J. Biol. Chem. 268:6269-6280.